Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 60058-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLOD3 Monoclonal Antibody (2G12B8)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: HEPHIADSW PQLQDHFSAV KLVGPEEALS PGEARDMAMD LCRQDPECEF YFSLDADAVL TNLQTLRILI EENRKVIAPM LSRHGKLWSN FWGALSPDEY YARSEDYVEL VQRKRVGVWN VPYISQAYVI RGDTLRMELP QRDVFSGSDT DPDMAFCKSF RDKGIFLHLS NQHEFGRLLA TSRYDTEHLH PDLWQIFDNP VDWKEQYIHE NYSRALEGEG IVEQPCPDVY WFPLLSEQMC DELVAEMEHY GQWSGGRHED SRLAGGYENV PTVDIHMKQV GYEDQWLQLL RTYVGPMTES LFPGYHTKAR AVMNFVVRYR PDEQPSLRPH HDSSTFTLNV ALNHKGLDYE GGGCRFLRYD CVISSPRKGW ALLHPGRLTH YHEGLPTTWG TRYIMVSFVD P (339-738 aa encoded by BC011674)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G12B8
- Vial size
- 150 µL
- Concentration
- 1.6 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human placenta tissue were subjected to SDS PAGE followed by western blot with 60058-1-IG (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of PLOD3 antibody (60058-1-IG, 1:2000) with si-Control and si-PLOD3 transfected HepG2 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using (Product # 60058-1-IG) (PLOD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 60058-1-IG (PLOD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IGG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer using 60058-1-IG (PLOD3 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer using 60058-1-IG (PLOD3 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PLOD3 (IP:60058-1-IG, 5ug; Detection:60058-1-IG 1:500) with HeLa cells lysate 1400ug.