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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- PA5-47271 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LGMN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Asparagine endopeptidase cleaves tau at N167 after uptake into microglia.
Behrendt A, Bichmann M, Ercan-Herbst E, Haberkant P, Schöndorf DC, Wolf M, Fahim SA, Murolo E, Ehrnhoefer DE
Neurobiology of disease 2019 Oct;130:104518
Neurobiology of disease 2019 Oct;130:104518
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human kidney tissue, human heart tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-human Legumain/Asparaginyl Endopeptidase Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47271) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. Specific bands were detected for Pro-Legumain/Asparaginyl Endopeptidase at approximately 56 kDa and mature Legumain/Asparaginyl Endopeptidase 37 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of LGMN in human kidney tissue, human heart tissue, and human placenta tissue. Samples were incubated in LGMN polyclonal antibody (Product # PA5-47271) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. Specific bands were detected for Pro-Legumain/Asparaginyl Endopeptidase at approximately 56 kDa and mature Legumain/Asparaginyl Endopeptidase 37 kDa (as indicated). This experiment was conducted under reducing conditions.
