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- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [8]
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- Product number
- HPA026744 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA026744, RRID:AB_2194642
- Product name
- Anti-SPACA1
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
REVILTNGCPGGESKCVVRVEECRGPTDCGWGKPI
SESLESVRLACIHTSPLNRFKYMWKL- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Submitted references Case report of apoptosis in testis of four AZFc-deleted patients: increased DNA fragmentation during meiosis, but decreased apoptotic markers in post-meiotic germ cells
Complete deletion of the AZFb interval from the Y chromosome in an oligozoospermic man
Streichemberger E, Perrin J, Saias-Magnan J, Karsenty G, Malzac P, Grillo J, Mitchell M, Metzler-Guillemain C
Human Reproduction 2012 June;27(7):1939-1945
Human Reproduction 2012 June;27(7):1939-1945
Complete deletion of the AZFb interval from the Y chromosome in an oligozoospermic man
Longepied G, Saut N, Aknin-Seifer I, Levy R, Frances A, Metzler-Guillemain C, Guichaoua M, Mitchell M
Human Reproduction 2010 September;25(10):2655-2663
Human Reproduction 2010 September;25(10):2655-2663
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and SPACA1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410624).
Enhanced validation
Enhanced validation
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human testis and endometrium tissues using Anti-SPACA1 antibody. Corresponding SPACA1 RNA-seq data are presented for the same tissues.
- Sample type
- HUMAN
Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Independent antibody validation
- Main image
- Experimental details
- Immunohistochemical staining of human cerebral cortex, kidney, liver and testis using Anti-SPACA1 antibody HPA026744 (A) shows similar protein distribution across tissues to independent antibody HPA043297 (B).
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human testis shows strong cytoplasmic positivity in spermatids and spermatozoa.
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human testis shows high expression.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human endometrium shows low expression as expected.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human kidney using Anti-SPACA1 antibody HPA026744.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human cerebral cortex using Anti-SPACA1 antibody HPA026744.
- Sample type
- HUMAN
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human liver using Anti-SPACA1 antibody HPA026744.
- Sample type
- HUMAN
