Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-89463 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89463, RRID:AB_11021011
- Product name
- Rabbit Polyclonal XRCC4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human XRCC4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair.
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Reid DA, Keegan S, Leo-Macias A, Watanabe G, Strande NT, Chang HH, Oksuz BA, Fenyo D, Lieber MR, Ramsden DA, Rothenberg E
Proceedings of the National Academy of Sciences of the United States of America 2015 May 19;112(20):E2575-84
Proceedings of the National Academy of Sciences of the United States of America 2015 May 19;112(20):E2575-84
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: XRCC4 Antibody [NBP1-89463] - Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: XRCC4 Antibody [NBP1-89463] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-XRCC4 antibody. Remaining relative intensity is presented
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: XRCC4 Antibody [NBP1-89463] - Analysis in control (vector only transfected HEK293T lysate) and XRCC4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-89463] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-89463] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: XRCC4 Antibody [NBP1-89463] - Staining in human testis and liver tissues using anti-XRCC4 antibody. Corresponding XRCC4 RNA-seq data are presented for the same tissues.