Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21569 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Adenylate Kinase 4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Raji. Predicted reactivity: Mouse (89%), Rat (88%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Adenylate Kinase 4 Polyclonal Antibody (Product # PA5-21569). Sample (30 µg whole cell lysate). A: 293T. B: A431. C: H1299. D: HeLa S3. E: Hep G2. F: Raji. 12% SDS PAGE. Adenylate Kinase 4 Polyclonal Antibody (Product # PA5-21569) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Adenylate Kinase 4 Polyclonal Antibody detects Adenylate kinase 4 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: Adenylate kinase 4 protein stained by Adenylate Kinase 4 Polyclonal Antibody (Product # PA5-21569) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded gastric cancer N87 xenograft, using Adenylate kinase 4 (Product # PA5-21569) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.