Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 46-1269-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD126 Monoclonal Antibody (47.7G7.1F2), PerCP-eFluor™ 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This monoclonal antibody 47.7G7.1F2 reacts with human CD126, also known as IL-6 receptor alpha. It associates with the gp130 chain to form the high-affinity receptor for IL-6. Although gp130 is ubiquitously expressed, CD126 is only found on mature T cells, activated B cells, macrophages, hepatocytes, and myeloma cells. Upon binding to IL-6, two heterotrimeric complexes of receptor and ligand associate into a hexamer and are then internalized, initializing Jak/STAT signaling pathways. IL-6 induces the proliferation and survival of T cells, Th2 differentiation, and acute-phase protein production in the liver. A soluble form of CD126 lacking the transmembrane domain is also present and can be generated by cleavage at the cell surface or alternative splicing. Soluble CD126 can bind IL-6 and activate cells expressing only gp130 through trans-presentation. Applications Reported: This 47.7G7.1F2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 47.7G7.1F2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 47.7G7.1F2
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Direct comparison of different therapeutic cell types susceptibility to inflammatory cytokines associated with COVID-19 acute lung injury.
The effects of IL-6 on CD4 T cell responses.
Vaka R, Khan S, Ye B, Risha Y, Parent S, Courtman D, Stewart DJ, Davis DR
Stem cell research & therapy 2022 Jan 15;13(1):20
Stem cell research & therapy 2022 Jan 15;13(1):20
The effects of IL-6 on CD4 T cell responses.
Dienz O, Rincon M
Clinical immunology (Orlando, Fla.) 2009 Jan;130(1):27-33
Clinical immunology (Orlando, Fla.) 2009 Jan;130(1):27-33
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0037-42) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human CD126 PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.