Antibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 21066-1-AP - Provider product page
- Provider
- Proteintech Group
- Product name
- MLKL antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated MLKL antibody (Cat. #21066-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Mixed lineage kinase domain-like pseudokinase-mediated necroptosis aggravates periodontitis progression.
Pterostilbene attenuates RIPK3-dependent hepatocyte necroptosis in alcoholic liver disease via SIRT2-mediated NFATc4 deacetylation.
Selenium Deficiency Induces Apoptosis and Necroptosis Through ROS/MAPK Signal in Human Uterine Smooth Muscle Cells.
Pterocephin A, a novel Triterpenoid Saponin from Pterocephalus hookeri induced liver injury by activation of necroptosis.
Role of HMGB1 in TNF-α Combined with Z-VAD-fmk-Induced L929 Cells Necroptosis.
Toxoplasma gondii secreted effectors co-opt host repressor complexes to inhibit necroptosis.
STAT3β Enhances Sensitivity to Concurrent Chemoradiotherapy by Inducing Cellular Necroptosis in Esophageal Squamous Cell Carcinoma.
Self-assembled polymeric nanocarrier-mediated co-delivery of metformin and doxorubicin for melanoma therapy.
Traditional herbal formula Wu-Mei-Wan alleviates TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation.
Cadmium exposure induces TNF-α-mediated necroptosis via FPR2/TGF-β/NF-κB pathway in swine myocardium.
RIP1/RIP3/MLKL mediates dopaminergic neuron necroptosis in a mouse model of Parkinson disease.
New insights in acetaminophen toxicity: HMGB1 contributes by itself to amplify hepatocyte necrosis in vitro through the TLR4-TRIF-RIPK3 axis.
Norfloxacin induces apoptosis and necroptosis in human corneal epithelial cells.
A necroptotic-independent function of MLKL in regulating endothelial cell adhesion molecule expression.
Quetiapine induces myocardial necroptotic cell death through bidirectional regulation of cannabinoid receptors.
FKBP12 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation.
Dexmedetomidine Preconditioning Protects Cardiomyocytes Against Hypoxia/Reoxygenation-Induced Necroptosis by Inhibiting HMGB1-Mediated Inflammation.
Visfatin Plays a Significant Role in Alleviating Lipopolysaccharide-Induced Apoptosis and Autophagy Through PI3K/AKT Signaling Pathway During Acute Lung Injury in Mice.
Neferine, a Bisbenzylisoquinoline Alkaloid, Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis.
Necrostatin-1 promotes ectopic periodontal tissue like structure regeneration in LPS-treated PDLSCs.
Receptor-Interacting Protein 3/Caspase-8 May Regulate Inflammatory Response and Promote Tissue Regeneration in the Periodontal Microenvironment.
Dihydrotanshinone I, a natural product, ameliorates DSS-induced experimental ulcerative colitis in mice.
Neuroprotective Effect of β-Caryophyllene on Cerebral Ischemia-Reperfusion Injury via Regulation of Necroptotic Neuronal Death and Inflammation: In Vivo and in Vitro.
A novel sgRNA selection system for CRISPR-Cas9 in mammalian cells.
Yang Y, Wang L, Zhang H, Luo L
Journal of molecular medicine (Berlin, Germany) 2022 Jan;100(1):77-86
Journal of molecular medicine (Berlin, Germany) 2022 Jan;100(1):77-86
Pterostilbene attenuates RIPK3-dependent hepatocyte necroptosis in alcoholic liver disease via SIRT2-mediated NFATc4 deacetylation.
Shao Y, Wang X, Zhou Y, Jiang Y, Wu R, Lu C
Toxicology 2021 Sep;461:152923
Toxicology 2021 Sep;461:152923
Selenium Deficiency Induces Apoptosis and Necroptosis Through ROS/MAPK Signal in Human Uterine Smooth Muscle Cells.
Wang Y, Li X, Yao Y, Zhao X, Shi X, Cai Y
Biological trace element research 2021 Sep 4;
Biological trace element research 2021 Sep 4;
Pterocephin A, a novel Triterpenoid Saponin from Pterocephalus hookeri induced liver injury by activation of necroptosis.
Wang R, Wei L, Dong Z, Meng F, Wang G, Zhou S, Lan X, Liao Z, Chen M
Phytomedicine : international journal of phytotherapy and phytopharmacology 2021 May;85:153548
Phytomedicine : international journal of phytotherapy and phytopharmacology 2021 May;85:153548
Role of HMGB1 in TNF-α Combined with Z-VAD-fmk-Induced L929 Cells Necroptosis.
Yu C, Lei Z, Li X, Huang LH, Li ZQ, Zhu HW, Han D, Huang H, Yu X
Biochemical genetics 2021 Jul 29;
Biochemical genetics 2021 Jul 29;
Toxoplasma gondii secreted effectors co-opt host repressor complexes to inhibit necroptosis.
Rosenberg A, Sibley LD
Cell host & microbe 2021 Jul 14;29(7):1186-1198.e8
Cell host & microbe 2021 Jul 14;29(7):1186-1198.e8
STAT3β Enhances Sensitivity to Concurrent Chemoradiotherapy by Inducing Cellular Necroptosis in Esophageal Squamous Cell Carcinoma.
Zheng ZY, Yang PL, Luo W, Yu SX, Xu HY, Huang Y, Li RY, Chen Y, Xu XE, Liao LD, Wang SH, Huang HC, Li EM, Xu LY
Cancers 2021 Feb 21;13(4)
Cancers 2021 Feb 21;13(4)
Self-assembled polymeric nanocarrier-mediated co-delivery of metformin and doxorubicin for melanoma therapy.
Song M, Xia W, Tao Z, Zhu B, Zhang W, Liu C, Chen S
Drug delivery 2021 Dec;28(1):594-606
Drug delivery 2021 Dec;28(1):594-606
Traditional herbal formula Wu-Mei-Wan alleviates TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation.
Wu F, Shao Q, Cheng Z, Xiong X, Fang K, Zhao Y, Dong R, Xu L, Lu F, Chen G
Chinese medicine 2021 Aug 16;16(1):78
Chinese medicine 2021 Aug 16;16(1):78
Cadmium exposure induces TNF-α-mediated necroptosis via FPR2/TGF-β/NF-κB pathway in swine myocardium.
Zhao X, Wang S, Li X, Liu H, Xu S
Toxicology 2021 Apr 15;453:152733
Toxicology 2021 Apr 15;453:152733
RIP1/RIP3/MLKL mediates dopaminergic neuron necroptosis in a mouse model of Parkinson disease.
Lin QS, Chen P, Wang WX, Lin CC, Zhou Y, Yu LH, Lin YX, Xu YF, Kang DZ
Laboratory investigation; a journal of technical methods and pathology 2020 Mar;100(3):503-511
Laboratory investigation; a journal of technical methods and pathology 2020 Mar;100(3):503-511
New insights in acetaminophen toxicity: HMGB1 contributes by itself to amplify hepatocyte necrosis in vitro through the TLR4-TRIF-RIPK3 axis.
Minsart C, Liefferinckx C, Lemmers A, Dressen C, Quertinmont E, Leclercq I, Devière J, Moreau R, Gustot T
Scientific reports 2020 Mar 27;10(1):5557
Scientific reports 2020 Mar 27;10(1):5557
Norfloxacin induces apoptosis and necroptosis in human corneal epithelial cells.
Yang T, Fan TJ, Xu B
Toxicology in vitro : an international journal published in association with BIBRA 2020 Aug;66:104868
Toxicology in vitro : an international journal published in association with BIBRA 2020 Aug;66:104868
A necroptotic-independent function of MLKL in regulating endothelial cell adhesion molecule expression.
Dai J, Zhang C, Guo L, He H, Jiang K, Huang Y, Zhang X, Zhang H, Wei W, Zhang Y, Lu L, Hu J
Cell death & disease 2020 Apr 24;11(4):282
Cell death & disease 2020 Apr 24;11(4):282
Quetiapine induces myocardial necroptotic cell death through bidirectional regulation of cannabinoid receptors.
Li X, Peng Z, Zhou Y, Wang J, Lin X, Dong X, Liu X, Jiang J, Jiang Y, Li L
Toxicology letters 2019 Oct 1;313:77-90
Toxicology letters 2019 Oct 1;313:77-90
FKBP12 mediates necroptosis by initiating RIPK1-RIPK3-MLKL signal transduction in response to TNF receptor 1 ligation.
Wang Z, Feng J, Yu J, Chen G
Journal of cell science 2019 May 20;132(10)
Journal of cell science 2019 May 20;132(10)
Dexmedetomidine Preconditioning Protects Cardiomyocytes Against Hypoxia/Reoxygenation-Induced Necroptosis by Inhibiting HMGB1-Mediated Inflammation.
Chen J, Jiang Z, Zhou X, Sun X, Cao J, Liu Y, Wang X
Cardiovascular drugs and therapy 2019 Feb;33(1):45-54
Cardiovascular drugs and therapy 2019 Feb;33(1):45-54
Visfatin Plays a Significant Role in Alleviating Lipopolysaccharide-Induced Apoptosis and Autophagy Through PI3K/AKT Signaling Pathway During Acute Lung Injury in Mice.
Wu XT, Ansari AR, Pang XX, Li HZ, Zhang ZW, Luo Y, Arshad M, Song H
Archivum immunologiae et therapiae experimentalis 2019 Aug;67(4):249-261
Archivum immunologiae et therapiae experimentalis 2019 Aug;67(4):249-261
Neferine, a Bisbenzylisoquinoline Alkaloid, Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis.
Wu X, Guo Y, Min X, Pei L, Chen X
The American journal of Chinese medicine 2018;46(6):1263-1279
The American journal of Chinese medicine 2018;46(6):1263-1279
Necrostatin-1 promotes ectopic periodontal tissue like structure regeneration in LPS-treated PDLSCs.
Yan B, Zhang H, Dai T, Gu Y, Qiu X, Hu C, Liu Y, Wei K, Li D
PloS one 2018;13(11):e0207760
PloS one 2018;13(11):e0207760
Receptor-Interacting Protein 3/Caspase-8 May Regulate Inflammatory Response and Promote Tissue Regeneration in the Periodontal Microenvironment.
Yan B, Wei K, Hou L, Dai T, Gu Y, Qiu X, Chen J, Feng Y, Cheng H, Yu Z, Zhang Y, Zhang H, Li D
Medical science monitor : international medical journal of experimental and clinical research 2018 Jul 29;24:5247-5257
Medical science monitor : international medical journal of experimental and clinical research 2018 Jul 29;24:5247-5257
Dihydrotanshinone I, a natural product, ameliorates DSS-induced experimental ulcerative colitis in mice.
Guo Y, Wu X, Wu Q, Lu Y, Shi J, Chen X
Toxicology and applied pharmacology 2018 Apr 1;344:35-45
Toxicology and applied pharmacology 2018 Apr 1;344:35-45
Neuroprotective Effect of β-Caryophyllene on Cerebral Ischemia-Reperfusion Injury via Regulation of Necroptotic Neuronal Death and Inflammation: In Vivo and in Vitro.
Yang M, Lv Y, Tian X, Lou J, An R, Zhang Q, Li M, Xu L, Dong Z
Frontiers in neuroscience 2017;11:583
Frontiers in neuroscience 2017;11:583
A novel sgRNA selection system for CRISPR-Cas9 in mammalian cells.
Zhang H, Zhang X, Fan C, Xie Q, Xu C, Zhao Q, Liu Y, Wu X, Zhang H
Biochemical and biophysical research communications 2016 Mar 18;471(4):528-32
Biochemical and biophysical research communications 2016 Mar 18;471(4):528-32
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 21066-1-AP( MLKL Antibody) at dilution of 1:300
- Other comments
- WB