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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [2]
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- Product number
- NBP1-05970 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-05970, RRID:AB_1555329
- Product name
- Rabbit Polyclonal HERC2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HERC2 Antibody [NBP1-05970] - Detection of Mouse HERC2- Whole cell lysate (50 ug) from NIH3T3, TCMK-1, Renca, 4T1, and CT26.WT cells. Affinity purified rabbit anti-HERC2 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HERC2 Antibody [NBP1-05970] - Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-HERC2 antibody used for WB. HERC2 was also immunoprecipitated by another rabbit anti-HERC2 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HERC2 Antibody [NBP1-05970] - Detection of human HERC2 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HERC2 antibody NBP1-05970 (lot NBP1-05970-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HERC2 was also immunoprecipitated by a previous lot (lot NBP1-05970-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).
