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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunoprecipitation [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AM26632AF-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti CD170 / SIGLEC5
- Antibody type
- Monoclonal
- Antigen
- Mouse SiglecE transfected L cell
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- 8D2
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation of mouse SiglecE from 293T cells (1 and 2) and mouse SiglecE transfected cells (2 and 4) with rat IgG2b (1 and 2) or AM26632AF-N (3 and 4). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with HRP-Streptavidin.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometic analysis mouse SiglecE expression on mouse splenocytes. The staining intensity of rat IgG2b at 10 µg/ml (1) or AM26632AF-N at 5 µg/ml (2) and 10 µg/ml (3) are shown in the horizontal axis with CD11c staining on the vertical axis.
