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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF1072 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Siglec-5/Siglec-14 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Siglec-5/14 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Siglec-7 and rhSiglec-9 is observed and less than 1% cross-reactivity with rhSiglec-2 and rhSiglec-3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O15389- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Siglec expression on the surface of human, bull and ram sperm.
Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity.
Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs.
IL-1F5 mediates anti-inflammatory activity in the brain through induction of IL-4 following interaction with SIGIRR/TIR8.
Alkhodair K, Almhanna H, McGetrick J, Gedair S, Gallagher ME, Fernandez-Fuertes B, Tharmalingam T, Larsen PB, Fitzpatrick E, Lonergan P, Evans ACO, Carrington SD, Reid CJ
Reproduction (Cambridge, England) 2018 Apr;155(4):361-371
Reproduction (Cambridge, England) 2018 Apr;155(4):361-371
Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity.
Pepin M, Mezouar S, Pegon J, Muczynski V, Adam F, Bianchini EP, Bazaa A, Proulle V, Rupin A, Paysant J, Panicot-Dubois L, Christophe OD, Dubois C, Lenting PJ, Denis CV
Scientific reports 2016 Nov 28;6:37953
Scientific reports 2016 Nov 28;6:37953
Siglec-9 enhances IL-10 production in macrophages via tyrosine-based motifs.
Ando M, Tu W, Nishijima K, Iijima S
Biochemical and biophysical research communications 2008 May 9;369(3):878-83
Biochemical and biophysical research communications 2008 May 9;369(3):878-83
IL-1F5 mediates anti-inflammatory activity in the brain through induction of IL-4 following interaction with SIGIRR/TIR8.
Costelloe C, Watson M, Murphy A, McQuillan K, Loscher C, Armstrong ME, Garlanda C, Mantovani A, O'Neill LA, Mills KH, Lynch MA
Journal of neurochemistry 2008 Jun;105(5):1960-9
Journal of neurochemistry 2008 Jun;105(5):1960-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Siglec-5/14 in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Human Siglec-5/14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1072, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by Siglec-5 and Neutralization by Human Siglec-5/14 Antibody. Recombinant Human Siglec-5 Fc Chimera (Catalog # 1072-SL), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human Siglec-5 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Human Siglec-5/14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1072). The ND50 is typically 0.5-2.0 µg/mL.
