AF2365

antibody from R&D Systems

Targeting: HAVCR2 CD366, FLJ14428, Tim-3, TIM3, TIMD3

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

AF2365

Provider

R&D Systems

Product name

Human TIM-3 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human TIM-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TIM-3 is observed and less than 1% cross-reactivity with recombinant human TIM-1, rmTIM-1, and rmTIM-2 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q8TDQ0


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells.

Franchini DM, Lanvin O, Tosolini M, Patras de Campaigno E, Cammas A, Péricart S, Scarlata CM, Lebras M, Rossi C, Ligat L, Pont F, Arimondo PB, Laurent C, Ayyoub M, Despas F, Lapeyre-Mestre M, Millevoi S, Fournié JJ
Cell reports 2019 Jan 2;26(1):94-107.e7

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Galectin-9 as a Predictive Marker for the Onset of Immune-Related Adverse Effects Associated with Anti-CCR4 MoAb Therapy in Patients with Adult T Cell Leukemia.

Mohammed TO, Chagan-Yasutan H, Ashino Y, Nakayama W, Takahashi Y, Shimomura T, Fujimoto T, Watanabe Y, Niki T, Suzushima H, Hattori T
The Tohoku journal of experimental medicine 2017 Mar;241(3):201-208

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Secretion of IFN-γ Associated with Galectin-9 Production by Pleural Fluid Cells from a Patient with Extrapulmonary Tuberculosis.

Zhao J, Shiratori B, Chagan-Yasutan H, Matsumoto M, Niki T, Tanaka M, Takahashi Y, Usami O, Ashino Y, Hattori T
International journal of molecular sciences 2017 Jun 28;18(7)

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TIM-1 Promotes Hepatitis C Virus Cell Attachment and Infection.

Wang J, Qiao L, Hou Z, Luo G
Journal of virology 2017 Jan 15;91(2)

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Frontline Science: Tim-3-mediated dysfunctional engulfment of apoptotic cells in SLE.

Zhao D, Guo M, Liu B, Lin Q, Xie T, Zhang Q, Jia X, Shu Q, Liang X, Gao L, Ma C
Journal of leukocyte biology 2017 Dec;102(6):1313-1322

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Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9.

Schwartz JA, Clayton KL, Mujib S, Zhang H, Rahman AK, Liu J, Yue FY, Benko E, Kovacs C, Ostrowski MA
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 15;198(8):3181-3194

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Comprehensive analyses of tumor immunity: implications for cancer immunotherapy.

Li B, Severson E, Pignon JC, Zhao H, Li T, Novak J, Jiang P, Shen H, Aster JC, Rodig S, Signoretti S, Liu JS, Liu XS
Genome biology 2016 Aug 22;17(1):174

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Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression.

Clayton KL, Douglas-Vail MB, Nur-ur Rahman AK, Medcalf KE, Xie IY, Chew GM, Tandon R, Lanteri MC, Norris PJ, Deeks SG, Ndhlovu LC, Ostrowski MA
Journal of virology 2015 Apr;89(7):3723-36

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Characterizing functional domains for TIM-mediated enveloped virus entry.

Moller-Tank S, Albritton LM, Rennert PD, Maury W
Journal of virology 2014 Jun;88(12):6702-13

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T cell Ig and mucin domain-containing protein 3 is recruited to the immune synapse, disrupts stable synapse formation, and associates with receptor phosphatases.

Clayton KL, Haaland MS, Douglas-Vail MB, Mujib S, Chew GM, Ndhlovu LC, Ostrowski MA
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 15;192(2):782-91

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Tim-3 expression defines regulatory T cells in human tumors.

Yan J, Zhang Y, Zhang JP, Liang J, Li L, Zheng L
PloS one 2013;8(3):e58006

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Decreased galectin-9 and increased Tim-3 expression are related to poor prognosis in gastric cancer.

Jiang J, Jin MS, Kong F, Cao D, Ma HX, Jia Z, Wang YP, Suo J, Cao X
PloS one 2013;8(12):e81799

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Tim-3 negatively regulates IL-12 expression by monocytes in HCV infection.

Zhang Y, Ma CJ, Wang JM, Ji XJ, Wu XY, Jia ZS, Moorman JP, Yao ZQ
PloS one 2011;6(5):e19664

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Dysregulation of TIM-3-galectin-9 pathway in the cystic fibrosis airways.

Vega-Carrascal I, Reeves EP, Niki T, Arikawa T, McNally P, O'Neill SJ, Hirashima M, McElvaney NG
Journal of immunology (Baltimore, Md. : 1950) 2011 Mar 1;186(5):2897-909

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Beneficial effect of galectin 9 on rheumatoid arthritis by induction of apoptosis of synovial fibroblasts.

Seki M, Sakata KM, Oomizu S, Arikawa T, Sakata A, Ueno M, Nobumoto A, Niki T, Saita N, Ito K, Dai SY, Katoh S, Nishi N, Tsukano M, Ishikawa K, Yamauchi A, Kuchroo V, Hirashima M
Arthritis and rheumatism 2007 Dec;56(12):3968-76

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

TIM-3 in Human Tonsil. TIM-3 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human TIM-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2365) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and extracellular space. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.