H00023533-D01P

antibody from Abnova Corporation

Targeting: PIK3R5 p101, P101-PI3K

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00023533-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00023533-D01P, RRID:AB_1711061

Product name

PIK3R5 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human PIK3R5 protein.

Antigen sequence

MDSGYVEDSEESSSEWPWRRGSQERRGHRRPGQKF
IRIYKLFKSTSQLVLRRDSRSLEGSSDTALPLRRA
GSLCSPLDEPVSPPSRAQRSRSLPQPKLGTQLPSW
LLAPASRPQRRRPFLSGDEDPKASTLRVVVFGSDR
ISGKVARAYSNLRRLENNRPLLTRFFKLQFFYVPV
KRSHGTSPGACPPPRSQTPSPPTDSPRHASPGELG
TTPWEESTNDISHYLGMLDPWYERNVLGLMHLPPE
VLCQQSLKAEAQALEGSPTQLPILADMLLYYCRFA
ARPVLLQVYQTELTFITGEKTTEIFIHSLELGHSA
ATRAIKASGPGSKRLGIDGDREAVPLTLQIIYSKG
AISGRSRWSNLEKVCTSVNLNKACRKQEELDSSME
ALTLNLTEVVKRQNSKSKKGFNQISTSQIKVDKVQ
IIGSNSCPFAVCLDQDERKILQSVVRCEVSPCYKP
EKSDLSSPPQTPPDLPAQAAPDLCSLLCLPIMTFS
GALP


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PIK3R5 expression in transfected 293T cell line (H00023533-T02) by PIK3R5 MaxPab polyclonal antibody.Lane 1: PIK3R5 transfected lysate(54.90 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between PIK3R5 and PIK3CG. HeLa cells were stained with anti-PIK3R5 rabbit purified polyclonal 1:1200 and anti-PIK3CG mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)