Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
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- Product number
- PA5-76034 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOX2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Serum amyloid A-containing HDL binds adipocyte-derived versican and macrophage-derived biglycan, reducing its antiinflammatory properties.
Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model.
Han CY, Kang I, Omer M, Wang S, Wietecha T, Wight TN, Chait A
JCI insight 2020 Sep 24;5(20)
JCI insight 2020 Sep 24;5(20)
Diastolic dysfunction is initiated by cardiomyocyte impairment ahead of endothelial dysfunction due to increased oxidative stress and inflammation in an experimental prediabetes model.
Waddingham MT, Sonobe T, Tsuchimochi H, Edgley AJ, Sukumaran V, Chen YC, Hansra SS, Schwenke DO, Umetani K, Aoyama K, Yagi N, Kelly DJ, Gaderi S, Herwig M, Kolijn D, Mügge A, Paulus WJ, Ogo T, Shirai M, Hamdani N, Pearson JT
Journal of molecular and cellular cardiology 2019 Dec;137:119-131
Journal of molecular and cellular cardiology 2019 Dec;137:119-131
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOX2 in extracts of MCF7 cell lines. Samples were incubated with NOX2 polyclonal antibody (Product # PA5-76034).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Control HDL inhibits palmitate-induced inflammation in TG-elicited peritoneal macrophages. TG-elicited peritoneal macrophages were preexposed to HDL (50 mug protein/mL) for 6 hours, after which the HDL was removed, the cells were washed, and the macrophages were incubated with or without palmitate (50 mumol/L) or TNF-alpha (10 ng/mL) for 24 hours before measurement of Saa3 , Ccl2 , Bgn , and Vcan gene expression ( A ), LR content and ROS generation ( B ), and TLR4 and NOX2 translocation to LRs ( C ), as described in Methods. Results are plotted as the mean fluorescence intensity of each sample on the vertical axis ( B ). An antibody against caveolin-1 (CAV1) was used to stain LRs ( C ). Fractions 7 to 9 contain LRs and fractions 1 to 4 are non-LR-containing fractions. Data are representative of 3 independent experiments ( n = 4) with mean +- SEM. * P < 0.01 vs. control HDL. ** P < 0.01 vs. palmitate. # P < 0.01 vs. TNF-alpha. ANOVA and Bonferroni's post hoc test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 HDL from AgNO 3 -injected mice loses its ability to inhibit palmitate-induced macrophage inflammation, to suppress LR content and ROS generation, and to block the translocation of TLR4 and NOX2 into LRs in peritoneal macrophages. HDL was isolated from the plasma of AgNO 3 - or PBS-injected C57BL/6 mice. TG-elicited peritoneal macrophages isolated from control C57BL/6 ( A - C ) or Saa3 -/- ( A ) mice were preexposed to HDL (50 mug protein/mL) for 6 hours, after which the HDL was removed, the cells were washed, and the peritoneal macrophages were incubated with or without palmitate (50 mumol/L) for 24 hours before measurement of Saa3 , Ccl2 , Tnfa , Il1b , and Il6 gene expression ( A ); LR content and ROS generation ( B ); and TLR4 and NOX2 translocation to LRs ( C ). Data are representative of 3 independent experiments ( n = 4) with mean +- SEM. * P < 0.001 vs. control HDL. ** P < 0.001 vs. palmitate. ANOVA and Bonferroni's post hoc test.