Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [4]
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- Product number
- NBP1-41012-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-41012-0.1mg, RRID:AB_10005006
- Product name
- Mouse Monoclonal CYBB/NOX2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody recognises the heavy chain of cytochrome B245. It recognises the native as well as the denatured form of the protein
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
Clone NL7 binds amino acids 498-506
(EKDVITGLK), a cytosolic epitope o
n gp91phox not accessible on intact
neutrophils.- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Staining of human peripheral blood granulocytes with Mouse anti Human Cytochrome b-245 Heavy Chain. Permeabilised with Leucoperm
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Analysis using the PE conjugate of NBP1-41012. Staining of CYBB in human peripheral blood using anti-CYBB antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): CYBB/NOX2 Antibody (NL7) [NBP1-41012] - An intracellular stain was performed on THP-1 cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control(orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CYBB/NOX2 Antibody (NL7) [NBP1-41012] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on Daudi cells with CYBB/NOX2 (NL7) antibody NBP1-41012AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.