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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [19]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-77673 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-77673, RRID:AB_1085374
- Product name
- Peripheral Node Addressin Antibody
- Antibody type
- Monoclonal
- Antigen
- Mouse lymph node stromal cells
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgM
- Vial size
- 0.5 mg
- Concentration
- 0.5 mg/ml
Submitted references Remodeling of Metastatic Vasculature Reduces Lung Colonization and Sensitizes Overt Metastases to Immunotherapy.
Tertiary lymphoid organs in the inflammatory myopathy associated with PD-1 inhibitors.
Anti-IL-6 eluting immunomodulatory biomaterials prolong skin allograft survival.
Targeted delivery of immune therapeutics to lymph nodes prolongs cardiac allograft survival.
Ectopic high endothelial venules in pancreatic ductal adenocarcinoma: A unique site for targeted delivery.
Lymphoid neogenesis in skin of human hand, nonhuman primate, and rat vascularized composite allografts.
Novel anti-carbohydrate antibodies reveal the cooperative function of sulfated N- and O-glycans in lymphocyte homing.
Aberrant endometrial features of pregnancy in diabetic NOD mice.
Ectopic LT alpha beta directs lymphoid organ neogenesis with concomitant expression of peripheral node addressin and a HEV-restricted sulfotransferase.
Human endomucin: distribution pattern, expression on high endothelial venules, and decoration with the MECA-79 epitope.
Nasal-associated lymphoid tissue: phenotypic and functional evidence for the primary role of peripheral node addressin in naive lymphocyte adhesion to high endothelial venules in a mucosal site.
Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody.
Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody.
The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing.
L-selectin-mediated lymphocyte rolling on MAdCAM-1.
The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor.
The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor.
Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes.
Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes.
He B, Johansson-Percival A, Backhouse J, Li J, Lee GYF, Hamzah J, Ganss R
Cell reports 2020 Jan 21;30(3):714-724.e5
Cell reports 2020 Jan 21;30(3):714-724.e5
Tertiary lymphoid organs in the inflammatory myopathy associated with PD-1 inhibitors.
Matsubara S, Seki M, Suzuki S, Komori T, Takamori M
Journal for immunotherapy of cancer 2019 Sep 18;7(1):256
Journal for immunotherapy of cancer 2019 Sep 18;7(1):256
Anti-IL-6 eluting immunomodulatory biomaterials prolong skin allograft survival.
Uehara M, Li X, Sheikhi A, Zandi N, Walker B, Saleh B, Banouni N, Jiang L, Ordikhani F, Dai L, Yonar M, Vohra I, Kasinath V, Orgill DP, Khademhosseini A, Annabi N, Abdi R
Scientific reports 2019 Apr 25;9(1):6535
Scientific reports 2019 Apr 25;9(1):6535
Targeted delivery of immune therapeutics to lymph nodes prolongs cardiac allograft survival.
Bahmani B, Uehara M, Jiang L, Ordikhani F, Banouni N, Ichimura T, Solhjou Z, Furtmüller GJ, Brandacher G, Alvarez D, von Andrian UH, Uchimura K, Xu Q, Vohra I, Yilmam OA, Haik Y, Azzi J, Kasinath V, Bromberg JS, McGrath MM, Abdi R
The Journal of clinical investigation 2018 Nov 1;128(11):4770-4786
The Journal of clinical investigation 2018 Nov 1;128(11):4770-4786
Ectopic high endothelial venules in pancreatic ductal adenocarcinoma: A unique site for targeted delivery.
Bahmani B, Uehara M, Ordikhani F, Li X, Jiang L, Banouni N, Ichimura T, Kasinath V, Eskandari SK, Annabi N, Bromberg JS, Shultz LD, Greiner DL, Abdi R
EBioMedicine 2018 Dec;38:79-88
EBioMedicine 2018 Dec;38:79-88
Lymphoid neogenesis in skin of human hand, nonhuman primate, and rat vascularized composite allografts.
Hautz T, Zelger BG, Nasr IW, Mundinger GS, Barth RN, Rodriguez ED, Brandacher G, Weissenbacher A, Zelger B, Cavadas P, Margreiter R, Lee WP, Pratschke J, Lakkis FG, Schneeberger S
Transplant international : official journal of the European Society for Organ Transplantation 2014 Sep;27(9):966-76
Transplant international : official journal of the European Society for Organ Transplantation 2014 Sep;27(9):966-76
Novel anti-carbohydrate antibodies reveal the cooperative function of sulfated N- and O-glycans in lymphocyte homing.
Hirakawa J, Tsuboi K, Sato K, Kobayashi M, Watanabe S, Takakura A, Imai Y, Ito Y, Fukuda M, Kawashima H
The Journal of biological chemistry 2010 Dec 24;285(52):40864-78
The Journal of biological chemistry 2010 Dec 24;285(52):40864-78
Aberrant endometrial features of pregnancy in diabetic NOD mice.
Burke SD, Dong H, Hazan AD, Croy BA
Diabetes 2007 Dec;56(12):2919-26
Diabetes 2007 Dec;56(12):2919-26
Ectopic LT alpha beta directs lymphoid organ neogenesis with concomitant expression of peripheral node addressin and a HEV-restricted sulfotransferase.
Drayton DL, Ying X, Lee J, Lesslauer W, Ruddle NH
The Journal of experimental medicine 2003 May 5;197(9):1153-63
The Journal of experimental medicine 2003 May 5;197(9):1153-63
Human endomucin: distribution pattern, expression on high endothelial venules, and decoration with the MECA-79 epitope.
Samulowitz U, Kuhn A, Brachtendorf G, Nawroth R, Braun A, Bankfalvi A, Böcker W, Vestweber D
The American journal of pathology 2002 May;160(5):1669-81
The American journal of pathology 2002 May;160(5):1669-81
Nasal-associated lymphoid tissue: phenotypic and functional evidence for the primary role of peripheral node addressin in naive lymphocyte adhesion to high endothelial venules in a mucosal site.
Csencsits KL, Jutila MA, Pascual DW
Journal of immunology (Baltimore, Md. : 1950) 1999 Aug 1;163(3):1382-9
Journal of immunology (Baltimore, Md. : 1950) 1999 Aug 1;163(3):1382-9
Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody.
Hemmerich S, Butcher EC, Rosen SD
The Journal of experimental medicine 1994 Dec 1;180(6):2219-26
The Journal of experimental medicine 1994 Dec 1;180(6):2219-26
Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody.
Hemmerich S, Butcher EC, Rosen SD
The Journal of experimental medicine 1994 Dec 1;180(6):2219-26
The Journal of experimental medicine 1994 Dec 1;180(6):2219-26
The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing.
Michie SA, Streeter PR, Bolt PA, Butcher EC, Picker LJ
The American journal of pathology 1993 Dec;143(6):1688-98
The American journal of pathology 1993 Dec;143(6):1688-98
L-selectin-mediated lymphocyte rolling on MAdCAM-1.
Berg EL, McEvoy LM, Berlin C, Bargatze RF, Butcher EC
Nature 1993 Dec 16;366(6456):695-8
Nature 1993 Dec 16;366(6456):695-8
The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor.
Berg EL, Robinson MK, Warnock RA, Butcher EC
The Journal of cell biology 1991 Jul;114(2):343-9
The Journal of cell biology 1991 Jul;114(2):343-9
The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor.
Berg EL, Robinson MK, Warnock RA, Butcher EC
The Journal of cell biology 1991 Jul;114(2):343-9
The Journal of cell biology 1991 Jul;114(2):343-9
Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes.
Streeter PR, Rouse BT, Butcher EC
The Journal of cell biology 1988 Nov;107(5):1853-62
The Journal of cell biology 1988 Nov;107(5):1853-62
Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes.
Streeter PR, Rouse BT, Butcher EC
The Journal of cell biology 1988 Nov;107(5):1853-62
The Journal of cell biology 1988 Nov;107(5):1853-62
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Peripheral Node Addressin Antibody (MECA-79) [NB100-77673] - Total protein from Human tonsil (lane 1) and human lymph node (lane 2) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/mL anti-PNAd in 1% milk, and detected with an anti-rat IgM HRP conjugated secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Peripheral Node Addressin Antibody (MECA-79) [NB100-77673] - Analysis of FFPE mouse adipose tissue section (with lymph node areas) using Peripheral Node Addressin antibody (clone MECA-79) at 1:100. The staining was developed with HRP-DAB detection method and the counterstaining was performed using hematoxylin. This Peripheral Node Addressin antibody generated a strong and specific staining of MECA-79 antigen in the the cytoplasm and the membranes of high endothelial venules (HEVs) aka peripheral lymph node addressin (PNAd) in lymph node areas of tested adipose tissue section.
