LS-C358743

antibody from LSBio

Targeting: NOD2 BLAU, CARD15, CD, CLR16.3, IBD1, NLRC2, PSORAS1

Western blot (Enhanced validation)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C358743

Provider

LSBio

Product name

NOD2 / CARD15 Antibody LS-C358743

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Human

Host

Rabbit

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Fig-1: Western blot analysis of NOD2 using at 1:2000. 30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M (lung carcinoma), SF-268 (glioblastoma), UO-31 and A498 (renal/kidney carcinoma), SK-MEL-5 and SK-MEL-28 (melanoma), and MCF-7 (breast carcinoma). Lymphoma is from a cell line generated from a tumor growing in nude mice following injection of primary human lymphoma cells.

Immunohistochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Fig-2: Formalin-fixed, paraffin-embedded normal human colon stained for NOD2 expression using at 1:2000. A, tissue core from a colon tissue microarray. A1, high magnification of A. Hematoxylin-eosin counterstain.