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LearnNB100-524
antibody from Novus Biologicals
Targeting: NOD2
BLAU, CARD15, CD, CLR16.3, IBD1, NLRC2, PSORAS1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [2]
- Flow cytometry [4]
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- Product number
- NB100-524 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-524, RRID:AB_10601840
- Product name
- Mouse Monoclonal NOD2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references TRIM22 inhibits endometrial cancer progression through the NOD2/NF‑κB signaling pathway and confers a favorable prognosis.
Immune disruption occurs through altered gut microbiome and NOD2 in arsenic induced mice: Correlation with colon cancer markers.
Nicotine modulates molecules of the innate immune response in epithelial cells and macrophages during infection with M. tuberculosis.
NOD1 and NOD2 of the innate immune system is differently expressed in human clear cell renal cell carcinoma, corresponding healthy renal tissue, its vasculature and primary isolated renal tubular epithelial cells.
The synergistic effect of NOD2 and TLR4 on the activation of autophagy in human submandibular gland inflammation.
Missense variants in NOX1 and p22phox in a case of very-early-onset inflammatory bowel disease are functionally linked to NOD2.
Parkin targets NOD2 to regulate astrocyte endoplasmic reticulum stress and inflammation.
Vitamin D Promotes Pneumococcal Killing and Modulates Inflammatory Responses in Primary Human Neutrophils.
Activation of nucleotide oligomerization domain 2 (NOD2) by human cytomegalovirus initiates innate immune responses and restricts virus replication.
RNAi screening identifies mediators of NOD2 signaling: implications for spatial specificity of MDP recognition.
Functional expression of NOD2 in freshly isolated human peripheral blood γδ T cells.
Genome-wide expression profiling identifies an impairment of negative feedback signals in the Crohn's disease-associated NOD2 variant L1007fsinsC.
Caspase recruitment domain-containing protein 8 (CARD8) negatively regulates NOD2-mediated signaling.
NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation.
DUOX2-derived reactive oxygen species are effectors of NOD2-mediated antibacterial responses.
NOD2 plays an important role in the inflammatory responses of microglia and astrocytes to bacterial CNS pathogens.
Altered host:pathogen interactions conferred by the Blau syndrome mutation of NOD2.
A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling.
Expression of NOD2 in Paneth cells: a possible link to Crohn's ileitis.
Expression of NOD2 in Paneth cells: a possible link to Crohn's ileitis.
Crohn's disease and the NOD2 gene: a role for paneth cells.
Crohn's disease and the NOD2 gene: a role for paneth cells.
Zhang L, Zhang B, Wei M, Xu Z, Kong W, Deng K, Xu X, Zhang L, Ζhao X, Yan L
International journal of oncology 2020 May;56(5):1225-1239
International journal of oncology 2020 May;56(5):1225-1239
Immune disruption occurs through altered gut microbiome and NOD2 in arsenic induced mice: Correlation with colon cancer markers.
Tikka C, Manthari RK, Ommati MM, Niu R, Sun Z, Zhang J, Wang J
Chemosphere 2020 May;246:125791
Chemosphere 2020 May;246:125791
Nicotine modulates molecules of the innate immune response in epithelial cells and macrophages during infection with M. tuberculosis.
Valdez-Miramontes CE, Trejo Martínez LA, Torres-Juárez F, Rodríguez Carlos A, Marin-Luévano SP, de Haro-Acosta JP, Enciso-Moreno JA, Rivas-Santiago B
Clinical and experimental immunology 2020 Feb;199(2):230-243
Clinical and experimental immunology 2020 Feb;199(2):230-243
NOD1 and NOD2 of the innate immune system is differently expressed in human clear cell renal cell carcinoma, corresponding healthy renal tissue, its vasculature and primary isolated renal tubular epithelial cells.
Mey L, Jung M, Roos F, Blaheta R, Hegele A, Kinscherf R, Urbschat A
Journal of cancer research and clinical oncology 2019 Jun;145(6):1405-1416
Journal of cancer research and clinical oncology 2019 Jun;145(6):1405-1416
The synergistic effect of NOD2 and TLR4 on the activation of autophagy in human submandibular gland inflammation.
Li J, Li B, Cheng Y, Meng Q, Wei L, Li W, Zhang J, Huang S
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2019 Jan;48(1):87-95
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2019 Jan;48(1):87-95
Missense variants in NOX1 and p22phox in a case of very-early-onset inflammatory bowel disease are functionally linked to NOD2.
Lipinski S, Petersen BS, Barann M, Piecyk A, Tran F, Mayr G, Jentzsch M, Aden K, Stengel ST, Klostermeier UC, Sheth V, Ellinghaus D, Rausch T, Korbel JO, Nothnagel M, Krawczak M, Gilissen C, Veltman JA, Forster M, Forster P, Lee CC, Fritscher-Ravens A, Schreiber S, Franke A, Rosenstiel P
Cold Spring Harbor molecular case studies 2019 Feb;5(1)
Cold Spring Harbor molecular case studies 2019 Feb;5(1)
Parkin targets NOD2 to regulate astrocyte endoplasmic reticulum stress and inflammation.
Singh K, Han K, Tilve S, Wu K, Geller HM, Sack MN
Glia 2018 Nov;66(11):2427-2437
Glia 2018 Nov;66(11):2427-2437
Vitamin D Promotes Pneumococcal Killing and Modulates Inflammatory Responses in Primary Human Neutrophils.
Subramanian K, Bergman P, Henriques-Normark B
Journal of innate immunity 2017;9(4):375-386
Journal of innate immunity 2017;9(4):375-386
Activation of nucleotide oligomerization domain 2 (NOD2) by human cytomegalovirus initiates innate immune responses and restricts virus replication.
Kapoor A, Forman M, Arav-Boger R
PloS one 2014;9(3):e92704
PloS one 2014;9(3):e92704
RNAi screening identifies mediators of NOD2 signaling: implications for spatial specificity of MDP recognition.
Lipinski S, Grabe N, Jacobs G, Billmann-Born S, Till A, Häsler R, Aden K, Paulsen M, Arlt A, Kraemer L, Hagemann N, Erdmann KS, Schreiber S, Rosenstiel P
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 26;109(52):21426-31
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 26;109(52):21426-31
Functional expression of NOD2 in freshly isolated human peripheral blood γδ T cells.
Marischen L, Wesch D, Oberg HH, Rosenstiel P, Trad A, Shomali M, Grötzinger J, Janssen O, Tchikov V, Schütze S, Kabelitz D
Scandinavian journal of immunology 2011 Aug;74(2):126-34
Scandinavian journal of immunology 2011 Aug;74(2):126-34
Genome-wide expression profiling identifies an impairment of negative feedback signals in the Crohn's disease-associated NOD2 variant L1007fsinsC.
Billmann-Born S, Till A, Arlt A, Lipinski S, Sina C, Latiano A, Annese V, Häsler R, Kerick M, Manke T, Seegert D, Hanidu A, Schäfer H, van Heel D, Li J, Schreiber S, Rosenstiel P
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4027-38
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4027-38
Caspase recruitment domain-containing protein 8 (CARD8) negatively regulates NOD2-mediated signaling.
von Kampen O, Lipinski S, Till A, Martin SJ, Nietfeld W, Lehrach H, Schreiber S, Rosenstiel P
The Journal of biological chemistry 2010 Jun 25;285(26):19921-6
The Journal of biological chemistry 2010 Jun 25;285(26):19921-6
NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation.
Cooney R, Baker J, Brain O, Danis B, Pichulik T, Allan P, Ferguson DJ, Campbell BJ, Jewell D, Simmons A
Nature medicine 2010 Jan;16(1):90-7
Nature medicine 2010 Jan;16(1):90-7
DUOX2-derived reactive oxygen species are effectors of NOD2-mediated antibacterial responses.
Lipinski S, Till A, Sina C, Arlt A, Grasberger H, Schreiber S, Rosenstiel P
Journal of cell science 2009 Oct 1;122(Pt 19):3522-30
Journal of cell science 2009 Oct 1;122(Pt 19):3522-30
NOD2 plays an important role in the inflammatory responses of microglia and astrocytes to bacterial CNS pathogens.
Chauhan VS, Sterka DG Jr, Furr SR, Young AB, Marriott I
Glia 2009 Mar;57(4):414-23
Glia 2009 Mar;57(4):414-23
Altered host:pathogen interactions conferred by the Blau syndrome mutation of NOD2.
Kim TH, Payne U, Zhang X, Iwanaga Y, Davey MP, Rosenbaum JT, Inman RD
Rheumatology international 2007 Jan;27(3):257-62
Rheumatology international 2007 Jan;27(3):257-62
A role for Erbin in the regulation of Nod2-dependent NF-kappaB signaling.
McDonald C, Chen FF, Ollendorff V, Ogura Y, Marchetto S, Lécine P, Borg JP, Nuñez G
The Journal of biological chemistry 2005 Dec 2;280(48):40301-9
The Journal of biological chemistry 2005 Dec 2;280(48):40301-9
Expression of NOD2 in Paneth cells: a possible link to Crohn's ileitis.
Ogura Y, Lala S, Xin W, Smith E, Dowds TA, Chen FF, Zimmermann E, Tretiakova M, Cho JH, Hart J, Greenson JK, Keshav S, Nuñez G
Gut 2003 Nov;52(11):1591-7
Gut 2003 Nov;52(11):1591-7
Expression of NOD2 in Paneth cells: a possible link to Crohn's ileitis.
Ogura Y, Lala S, Xin W, Smith E, Dowds TA, Chen FF, Zimmermann E, Tretiakova M, Cho JH, Hart J, Greenson JK, Keshav S, Nuñez G
Gut 2003 Nov;52(11):1591-7
Gut 2003 Nov;52(11):1591-7
Crohn's disease and the NOD2 gene: a role for paneth cells.
Lala S, Ogura Y, Osborne C, Hor SY, Bromfield A, Davies S, Ogunbiyi O, Nuñez G, Keshav S
Gastroenterology 2003 Jul;125(1):47-57
Gastroenterology 2003 Jul;125(1):47-57
Crohn's disease and the NOD2 gene: a role for paneth cells.
Lala S, Ogura Y, Osborne C, Hor SY, Bromfield A, Davies S, Ogunbiyi O, Nuñez G, Keshav S
Gastroenterology 2003 Jul;125(1):47-57
Gastroenterology 2003 Jul;125(1):47-57
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NOD2 Antibody (2D9) [NB100-524] - anti-NOD2 (2D9) Whole cell protein from THP-1 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-NOD2 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NOD2 Antibody (2D9) [NB100-524] - Detection of NOD2 in 20ug of NOD2 transfected 293T cell lysate using NB 100-524. ECL detection in 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NOD2 Antibody (2D9) [NB100-524] - Detection of endogenous NOD2 in 50ug of HT29 cell lysate using NB 100-524. ECL detection in 15 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NOD2 Antibody (2D9) [NB100-524] - Whole cell protein from THP-1 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-NOD2 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: NOD2 Antibody (2D9) [NB100-524] - Analysis of PFA fixed mouse sciatic nerve section using anti-NOD2 antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on THP-1 cells with NOD2 (2D9) antibody NB100-524AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Raji cells with NOD2 (2D9) antibody NB100-524AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.Both antibodies were conjugated to phycoerythrin.
