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LearnNB500-253
antibody from Novus Biologicals
Targeting: NOD2
BLAU, CARD15, CD, CLR16.3, IBD1, NLRC2, PSORAS1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB500-253 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-253, RRID:AB_10001869
- Product name
- Rabbit Polyclonal NOD2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.87 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Missense variants in NOX1 and p22phox in a case of very-early-onset inflammatory bowel disease are functionally linked to NOD2.
NOD1 and NOD2 regulate proinflammatory and prolabor mediators in human fetal membranes and myometrium via nuclear factor-kappa B.
RNAi screening identifies mediators of NOD2 signaling: implications for spatial specificity of MDP recognition.
A role for membrane-bound CD147 in NOD2-mediated recognition of bacterial cytoinvasion.
Lipinski S, Petersen BS, Barann M, Piecyk A, Tran F, Mayr G, Jentzsch M, Aden K, Stengel ST, Klostermeier UC, Sheth V, Ellinghaus D, Rausch T, Korbel JO, Nothnagel M, Krawczak M, Gilissen C, Veltman JA, Forster M, Forster P, Lee CC, Fritscher-Ravens A, Schreiber S, Franke A, Rosenstiel P
Cold Spring Harbor molecular case studies 2019 Feb;5(1)
Cold Spring Harbor molecular case studies 2019 Feb;5(1)
NOD1 and NOD2 regulate proinflammatory and prolabor mediators in human fetal membranes and myometrium via nuclear factor-kappa B.
Lappas M
Biology of reproduction 2013 Jul;89(1):14
Biology of reproduction 2013 Jul;89(1):14
RNAi screening identifies mediators of NOD2 signaling: implications for spatial specificity of MDP recognition.
Lipinski S, Grabe N, Jacobs G, Billmann-Born S, Till A, Häsler R, Aden K, Paulsen M, Arlt A, Kraemer L, Hagemann N, Erdmann KS, Schreiber S, Rosenstiel P
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 26;109(52):21426-31
Proceedings of the National Academy of Sciences of the United States of America 2012 Dec 26;109(52):21426-31
A role for membrane-bound CD147 in NOD2-mediated recognition of bacterial cytoinvasion.
Till A, Rosenstiel P, Bräutigam K, Sina C, Jacobs G, Oberg HH, Seegert D, Chakraborty T, Schreiber S
Journal of cell science 2008 Feb 15;121(Pt 4):487-95
Journal of cell science 2008 Feb 15;121(Pt 4):487-95
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NOD2 Antibody [NB500-253] - Detection of NOD2 in 20 ug of NOD2 transfected 293T cell lysate using NB 500-253. ECL detection in 15 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NOD2 Antibody [NB500-253] - Analysis of FFPE human skin using NOD2 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of NOD2 was observed. Staining was performed by Histowiz.
