BS4051

antibody from Bioworld Technology, Inc
Targeting: MAP3K8 c-COT, COT, EST, ESTF, MEKK8, Tpl-2
Provider product page for BS4051
Western blot
Immunocytochemistry
Immunohistochemistry

Antibody data

Product number
BS4051 - Provider product page
Provider
Bioworld Technology, Inc
Product name
Cot (phospho T290) polyclonal antibody
Antibody type
Polyclonal
Antigen
Synthetic phosphopeptide derived from human Cot around the phosphorylation site of Threonine 290.
Description
The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1.
Reactivity
Human, Mouse, Rat
Host
Rabbit
Isotype
IgG
Vial size
100ul
Concentration
1 mg/ml
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
MAP3K8 protein structure - BS4051 shown in red.