AP7913a

antibody from Abgent

Targeting: MAP3K8 c-COT, COT, EST, ESTF, MEKK8, Tpl-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7913a

Provider

Abgent

Proper citation

Abgent Cat#AP7913A, RRID:AB_2235009

Product name

COT (MAP3K8/MEKK8) Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

RB0947; RB0948

Vial size

400 µl

Storage

Store at 2 to 8°C.Antibody is stable for 28 months.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of MEKK8 (arrow) using MEKK8 Antibody (C-term) (Cat.#AP7913a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K8 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100