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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2282 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2282, RRID:AB_1577183
- Product name
- MAP3K8 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MAP3K8.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references COT kinase proto-oncogene expression in T cells: implication of the JNK/SAPK signal transduction pathway in COT promoter activation.
The human cot proto-oncogene encodes two protein serine/threonine kinases with different transforming activities by alternative initiation of translation.
Expression cDNA cloning of a serine kinase transforming gene.
Sánchez-Góngora E, Lisbona C, de Gregorio R, Ballester A, Calvo V, Pérez-Jurado L, Alemany S
The Journal of biological chemistry 2000 Oct 6;275(40):31379-86
The Journal of biological chemistry 2000 Oct 6;275(40):31379-86
The human cot proto-oncogene encodes two protein serine/threonine kinases with different transforming activities by alternative initiation of translation.
Aoki M, Hamada F, Sugimoto T, Sumida S, Akiyama T, Toyoshima K
The Journal of biological chemistry 1993 Oct 25;268(30):22723-32
The Journal of biological chemistry 1993 Oct 25;268(30):22723-32
Expression cDNA cloning of a serine kinase transforming gene.
Chan AM, Chedid M, McGovern ES, Popescu NC, Miki T, Aaronson SA
Oncogene 1993 May;8(5):1329-33
Oncogene 1993 May;8(5):1329-33
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MAP3K8 polyclonal antibody (Cat # PAB2282) in A-375 cell lysate. MAP3K8 (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the MAP3K8 polyclonal antibody (Cat # PAB2282) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
