HPA017962

antibody from Atlas Antibodies

Targeting: MAP3K8 c-COT, COT, EST, ESTF, MEKK8, Tpl-2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA017962

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA017962, RRID:AB_1853776

Product name

Anti-MAP3K8

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

ENLYASEEPAVYEPSLMTMCQDSNQNDERSKSLLL
SGQEVPWLSSVRYGTVEDLLAFANHISNTAKHFYG
QRPQESGILLNMVITPQNGRYQIDSDVLLIPWKLT
YRNIGSDFIPRGAFGKVYLAQDIKTKKRMACKLIP
VDQFKPSDV


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and MAP3K8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401593).

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells and non-germinal center cells.

Sample type

HUMAN