Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C344739 - Provider product page
- Provider
- LSBio
- Product name
- FTO Antibody (clone AG103) LS-C344739
- Antibody type
- Monoclonal
- Description
- Protein G purified from tissue culture supernatant
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AG103
- Storage
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
No comments: Submit comment
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of human FTO using anti-human FTO mAb (AG103) at 1:1,000 dilution:. 1. hFTO (His-tagged) 100 ng. 2. HEK293T 100 ug. 3. HEK293E 100 ug. 4. HepG2 100 ug. 5. Hep3B 50 ug. 6. VIT1 80 ug. 7. THP1 74 ug. 8. Molt4 96 ug. 9. A549 48 ug
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of recombinant human FTO proteins using anti-FTO (human), mAb (AG103) at different concentrations. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot with rabbit anti-mouse FTO pAb . 1. hFTO (His-tagged) recombinant control 100ng (AG-40A-112). 2. hFTO (His-tagged) 5 ug. 3. hFTO (His-tagged) 2.5 ug. 4. hFTO (His-tagged) 1.25 ug. 5. hFTO (His-tagged) 0.625 ug. 6. hFTO (His-tagged) 0.312 ug. 7. hFTO (His-tagged) 0.156 ug. 8. hFTO (His-tagged) 0.078 ug
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemical staining of FTO with anti-FTO (human), mAb (AG103) in brain, hypothalamus and paraventricular nucleus (5~10 ug/ml). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain (40x).
