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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-17527 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 4E-BP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 4E-BP2 in extracts from A673 cells, untreated or nocodazole-treated (100 ng/mL, 16 hrs), using 4E-BP2 polyclonal antibody (Product # PA5-17527). Extracts were treated with lambda phosphatase (10,000 U/mL for 1 hour) to dephosphorylate proteins.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 4E-BP2 in bacterially expressed GST-4E-BP1 and of extracts from NIH/3T3 cells using 4E-BP2 polyclonal antibody (Product # PA5-17527) and an alternate 4E-BP1 polyclonal antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of 4E-BP2 in paraffin-embedded human colon carcinoma using a 4E-BP2 polyclonal antibody (Product # PA5-17527) showing cytoplasmic and membrane localization.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of 4E-BP2 in HeLa cells using a 4E-BP2 polyclonal antibody (Product # PA5-17527) (blue) compared to a nonspecifc negative control antibody (red).
