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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP8154c - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP8154c, RRID:AB_2236955
- Product name
- PFKFB4 Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Sertoli-secreted FGF-2 induces PFKFB4 isozyme expression in mouse spermatogenic cells by activation of the MEK/ERK/CREB pathway.
Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases.
Ras inhibition in glioblastoma down-regulates hypoxia-inducible factor-1alpha, causing glycolysis shutdown and cell death.
Gómez M, Manzano A, Figueras A, Viñals F, Ventura F, Rosa JL, Bartrons R, Navarro-Sabaté À
American journal of physiology. Endocrinology and metabolism 2012 Sep 15;303(6):E695-707
American journal of physiology. Endocrinology and metabolism 2012 Sep 15;303(6):E695-707
Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases.
Yalcin A, Clem BF, Simmons A, Lane A, Nelson K, Clem AL, Brock E, Siow D, Wattenberg B, Telang S, Chesney J
The Journal of biological chemistry 2009 Sep 4;284(36):24223-32
The Journal of biological chemistry 2009 Sep 4;284(36):24223-32
Ras inhibition in glioblastoma down-regulates hypoxia-inducible factor-1alpha, causing glycolysis shutdown and cell death.
Blum R, Jacob-Hirsch J, Amariglio N, Rechavi G, Kloog Y
Cancer research 2005 Feb 1;65(3):999-1006
Cancer research 2005 Feb 1;65(3):999-1006
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- The anti-PFKFB4 Pab (Cat. #AP8154c) is used in Western blot to detect PFKFB4 in mouse brain tissue lysate.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB4 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
