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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- GTX107755 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107755, RRID:AB_1951183
- Product name
- PFKFB4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PFKFB4 antibody detects PFKFB4 protein by Western blot analysis.A. 50 µg mouse liver lysate/extract 10 % SDS-PAGEPFKFB4 antibody (GTX107755) dilution: 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PFKFB4 antibody detects PFKFB4 protein by Western blot analysis.A. 50 µg rat liver lysate/extract 10 % SDS-PAGEPFKFB4 antibody (GTX107755) dilution: 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g whole cell lysate)A:Raji (GTX27908)10% SDS PAGEGTX107755 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PFKFB4 antibody detects PFKFB4 protein at cytoplasm by immunofluorescent analysis.Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.Green: PFKFB4 protein stained by PFKFB4 antibody (GTX107755) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using PFKFB4(GTX107755) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PFKFB4 antibody detects PFKFB4 protein at cytoplasm on mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. PFKFB4 antibody (GTX107755) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PFKFB4 antibody detects PFKFB4 protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PFKFB4 antibody (GTX107755) diluted at 1:500.
