Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-15475 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PFKFB4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with non-human primate based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Discovery of Mitochondrial Transcription Inhibitors Active in Pancreatic Cancer Cells.
Synthesis and mechanistic studies of quinolin-chlorobenzothioate derivatives with proteasome inhibitory activity in pancreatic cancer cell lines.
Immunohistochemical analysis of PDK1, PHD3 and HIF-1α expression defines the hypoxic status of neuroblastoma tumors.
Chen W, Hu S, Mao S, Xu Y, Guo H, Li H, Paulsen MT, Chen X, Ljungman M, Neamati N
ChemMedChem 2020 Nov 4;15(21):2029-2039
ChemMedChem 2020 Nov 4;15(21):2029-2039
Synthesis and mechanistic studies of quinolin-chlorobenzothioate derivatives with proteasome inhibitory activity in pancreatic cancer cell lines.
Hu S, Jin Y, Liu Y, Ljungman M, Neamati N
European journal of medicinal chemistry 2018 Oct 5;158:884-895
European journal of medicinal chemistry 2018 Oct 5;158:884-895
Immunohistochemical analysis of PDK1, PHD3 and HIF-1α expression defines the hypoxic status of neuroblastoma tumors.
Ognibene M, Cangelosi D, Morini M, Segalerba D, Bosco MC, Sementa AR, Eva A, Varesio L
PloS one 2017;12(11):e0187206
PloS one 2017;12(11):e0187206
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PFKFB4 using a PFKFB4 polyclonal antibody (Product # PA5-15475) in mouse brain tissue lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using a PFKFB4 polyclonal antibody (Product # PA5-15475), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure SQD1 , Mito-Chlor , and H 2 O 2 induced similar changes in protein expression in MIA PaCa-2 cells. (A), 24 h treatment of SQD1 increased the expression levels of FOSB, c-JUN, HMOX1, HK2, PFKFB4, MAPLC-3alpha/beta, and GABARAPL in a dose-dependent manner. Mito-Chlor treatment induced similar protein expression changes with the exception of GABARAPL, HK2, and PFKFB4. SQD1 -, Mito-Chlor -, and H 2 O 2 -induced changes in select protein levels were rescued by the addition of 1.5 mM NAC. (B), Protein levels induced by SQD1 and Mito-Chlor were quantified by ImageJ and normalized to respective loading controls. The data are presented as mean+-standard deviation of at least three independent experiments. * denotes p
