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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- GTX105700 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX105700, RRID:AB_1950808
- Product name
- LGI1 antibody [N2C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate)A: A431 (GTX27909)7.5% SDS PAGEGTX105700 diluted at 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGI1 antibody [N2C2], Internal (GTX105700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LGI1 antibody [N2C2], Internal (GTX105700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- LGI1 antibody [N2C2], Internal detects LGI1 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: LGI1 protein stained by LGI1 antibody [N2C2], Internal (GTX105700) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
