14602-1-AP

antibody from Invitrogen Antibodies

Targeting: MAGEA6 CT1.6, MAGE-3b, MAGE3B, MAGE6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

14602-1-AP

Provider

Invitrogen Antibodies

Product name

MAGEA6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPLEQRSQH CKPEEGLEAR GEALGLVGAQ APATEEQEAA SSSSTLVEVT LGEVPAAESP DPPQSPQGAS SLPTTMNYPL WSQSYEDSSN QEEEGPSTFP DLESEFQAAL SRKVAKLVHF LLLKYRAREP VTKAEMLGSV VGNWQYFFPV IFIKASDSLQ LVFGIELMEV DPIGHVYIFA TCLGLSYDGL LGDNQIMPKT GFLIIILAII AKEGDCAPEE KIWEELSVLE VFEGREDSIF GDPKKLLTQY FVQENYLEYR QVPGSDPACY EFLWGPRALI ETSYVKVLHH MVKISGGPRI SYPLLHEWAL REGEE (1-314 aa encoded by BC067731)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.18 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 14602-1-AP (MAGEA6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14602-1-AP (MAGEA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 14602-1-AP (MAGEA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14602-1-AP (MAGEA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 14602-1-AP (MAGEA6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A375 cells using 14602-1-AP (MAGEA6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A375 cells using 14602-1-AP (MAGEA6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 14602-1-AP (MAGEA6 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 14602-1-AP (MAGEA6 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 14602-1-AP (MAGEA6 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 14602-1-AP (MAGEA6 antibody) at dilution of 1:50 (under 40x lens).