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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- 13385-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CPN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: RNQRIVQLI QDTRIHILPS MNPDGYEVAA AQGPNKPGYL VGRNNANGVD LNRNFPDLNT YIYYNEKYGG PNHHLPLPDN WKSQVEPETR AVIRWMHSFN FVLSANLHGG AVVANYPYDK SFEHRVRGVR RTASTPTPDD KLFQKLAKVY SYAHGWMFQG WNCGDYFPDG ITNGASWYSL SKGMQDFNYL HTNCFEITLE LSCDKFPPEE ELQREWLGNR EALIQFLEQV HQGIKGMVLD ENYNNLANAV ISVSGINHDV TSGDHGDYFR LLLPGIYTVS ATAPGYDPET VTVTVGPAEP TLVNFHLKRS IPQVSPVRRA PSRRHGVRAK VQPQARKKEM EMRQLQRGPA (110-458 aa encoded by BC027897)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 13385-1-AP (CPN1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 13385-1-AP (CPN1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 13385-1-AP (CPN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COLO 320 cells using 13385-1-AP (CPN1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 13385-1-AP (CPN1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 13385-1-AP (CPN1 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug CPN1 antibody (13385-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
