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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- GTX114161 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114161, RRID:AB_10619542
- Product name
- Rhotekin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Midbody localization of vinexin recruits rhotekin to facilitate cytokinetic abscission.
Chang YW, Huang YS
Cell cycle (Georgetown, Tex.) 2017;16(21):2046-2057
Cell cycle (Georgetown, Tex.) 2017;16(21):2046-2057
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX114161 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE Rhotekin antibody GTX114161 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rhotekin antibody detects Rhotekin protein by western blot analysis.A. 30 ?g Jurkat whole cell lysate/extract B. 30 ?g Raji whole cell lysate/extract C. 30 ?g NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGERhotekin antibody (GTX114161) dilution: 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rhotekin antibody detects Rhotekin protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of human Rhotekin-transfected 293T cells7.5 % SDS-PAGERhotekin antibody (GTX114161) dilution: 1:5000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rhotekin antibody detects Rhotekin protein at cytoplasm by immunofluorescent analysis.Sample: Rhotekin-transfected (right) or untransfected (left) HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Rhotekin protein stained by Rhotekin antibody (GTX114161) diluted at 1:500.Blue: Hoechst 33342 staining.
