Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00006242-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006242-M01, RRID:AB_2254385
- Product name
- Mouse Monoclonal RTKN Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RTKN (2E5)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Protein kinase D regulates RhoA activity via rhotekin phosphorylation.
Pseudopodial actin dynamics control epithelial-mesenchymal transition in metastatic cancer cells.
Pusapati GV, Eiseler T, Rykx A, Vandoninck S, Derua R, Waelkens E, Van Lint J, von Wichert G, Seufferlein T
The Journal of biological chemistry 2012 Mar 16;287(12):9473-83
The Journal of biological chemistry 2012 Mar 16;287(12):9473-83
Pseudopodial actin dynamics control epithelial-mesenchymal transition in metastatic cancer cells.
Shankar J, Messenberg A, Chan J, Underhill TM, Foster LJ, Nabi IR
Cancer research 2010 May 1;70(9):3780-90
Cancer research 2010 May 1;70(9):3780-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RTKN Antibody (2E5) [H00006242-M01] - RTKN monoclonal antibody (M01), clone 2E5 Analysis of RTKN expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RTKN Antibody (2E5) [H00006242-M01] - Detection limit for recombinant GST tagged RTKN is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RTKN Antibody (2E5) [H00006242-M01] - Analysis of monoclonal antibody to RTKN on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.