PA5-70630

antibody from Invitrogen Antibodies

Targeting: PROK2 BV8, KAL4, MIT1, PK2

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-70630

Provider

Invitrogen Antibodies

Product name

PROK2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This target displays homology in the following species: Cow: 85%; Human: 100%; Mouse: 79%; Pig: 86%; Rat: 86%

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

The novel function of miR-3195 for mutant PROK2 (c.223-4C>A) degradation.

Zhou S, Li P
Cell biology international 2021 Feb;45(2):404-410

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PROK2 on human HT1080 cells. The sample was probed with a PROK2 polyclonal antibody (Product # PA5-70630) using a primary antibody dilution of 1.0 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

3 Figure Experimental evidence that the mutant PROK2 is targeted by miR-3195. The mRNA and protein levels of PROK2 in 293T cells treated with miR-3195, miR-4497, and miR-7704 determined using qPCR (a), western blot (b), and immunofluorescence (c). BL, WT, and MT indicate blank, wild type, and mutant, respectively. The qPCR data are presented as the mean +- SEM of three separate experiments. ""**"" represents the difference between ""MT"" and ""WT"" with a p value less than .01 by one-way ANOVA. ANOVA, analysis of variance; mRNA, messenger RNA; miRNA, microRNA; PROK2, chemokine prokineticin-2; qPCR, quantitative polymerase chain reaction assay