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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- PA1-085 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-085 detects MAL from rat, mouse, xenopus and human samples.
- Concentration
- 1 mg/mL
Submitted references Mal protein stabilizes luminal membrane PLC-β3 and negatively regulates ENaC in mouse cortical collecting duct cells.
Tuna KM, Liu BC, Yue Q, Ghazi ZM, Ma HP, Eaton DC, Alli AA
American journal of physiology. Renal physiology 2019 Oct 1;317(4):F986-F995
American journal of physiology. Renal physiology 2019 Oct 1;317(4):F986-F995
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of MAL was performed by loading 50 µg of mouse mpkCCD and Xenopus 2f3 whole cell lysates per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% nonfat dry milk in TBS for 1 hour at room temperature. The membrane was probed with a MAL polyclonal antibody (Product # PA1-085) at a dilution of 1:500 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody for 1 hour. Detection was performed using SuperSignal West Dura (Product # 34075). Data courtesy of the Innovators Program.
