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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
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- Product number
- GTX112692 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112692, RRID:AB_11179007
- Product name
- Apolipoprotein A1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Isolation of High-Purity Extracellular Vesicles by the Combination of Iodixanol Density Gradient Ultracentrifugation and Bind-Elute Chromatography From Blood Plasma.
Onódi Z, Pelyhe C, Terézia Nagy C, Brenner GB, Almási L, Kittel Á, Manček-Keber M, Ferdinandy P, Buzás EI, Giricz Z
Frontiers in physiology 2018;9:1479
Frontiers in physiology 2018;9:1479
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE GTX112692 diluted at 1:2000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody (GTX112692) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody (GTX112692) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human tissue extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody (GTX112692) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human plasma (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody (GTX112692) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Apolipoprotein A1 antibody detects Apolipoprotein A1 protein at cytoplasm in human liver by immunohistochemical analysis. Sample: Paraffin-embedded human liver. Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
