Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB400-147 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB400-147, RRID:AB_10001123
- Product name
- Goat Polyclonal Apolipoprotein A-I/ApoA1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA.This reacts with human apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.2 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Submitted references Plasma Nitration of High-Density and Low-Density Lipoproteins in Chronic Kidney Disease Patients Receiving Kidney Transplants.
Robust passive and active efflux of cellular cholesterol to a designer functional mimic of high density lipoprotein.
Factors controlling nascent high-density lipoprotein particle heterogeneity: ATP-binding cassette transporter A1 activity and cell lipid and apolipoprotein AI availability.
Mechanism of ATP-binding cassette transporter A1-mediated cellular lipid efflux to apolipoprotein A-I and formation of high density lipoprotein particles.
Bakillah A, Tedla F, Ayoub I, John D, Norin AJ, Hussain MM, Brown C
Mediators of inflammation 2015;2015:352356
Mediators of inflammation 2015;2015:352356
Robust passive and active efflux of cellular cholesterol to a designer functional mimic of high density lipoprotein.
Luthi AJ, Lyssenko NN, Quach D, McMahon KM, Millar JS, Vickers KC, Rader DJ, Phillips MC, Mirkin CA, Thaxton CS
Journal of lipid research 2015 May;56(5):972-85
Journal of lipid research 2015 May;56(5):972-85
Factors controlling nascent high-density lipoprotein particle heterogeneity: ATP-binding cassette transporter A1 activity and cell lipid and apolipoprotein AI availability.
Lyssenko NN, Nickel M, Tang C, Phillips MC
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Jul;27(7):2880-92
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Jul;27(7):2880-92
Mechanism of ATP-binding cassette transporter A1-mediated cellular lipid efflux to apolipoprotein A-I and formation of high density lipoprotein particles.
Vedhachalam C, Duong PT, Nickel M, Nguyen D, Dhanasekaran P, Saito H, Rothblat GH, Lund-Katz S, Phillips MC
The Journal of biological chemistry 2007 Aug 24;282(34):25123-30
The Journal of biological chemistry 2007 Aug 24;282(34):25123-30
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - Coomassie stained gel showing both free and HDL bound apoA-I eluted from a solid phase resin prepared using anti-Human apolipoprotein A-I antibody. The resin was reacted with human serum prior to washing and elution of bound proteins. The gel was composed of 0.75% agarose in a native buffer system. Separation occurred at room temperature.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Apolipoprotein A-I/ApoA1 Antibody [NB400-147] - Immunohistochemistry. Immunochemicals anti-APOA1 antibody was used at a 5 ug/ml to detect signal in human liver tissue. Tissue was formalin-fixed and paraffin embedded.
