19140-1-AP

antibody from Invitrogen Antibodies

Targeting: VPS11 PEP5, RNF108

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

19140-1-AP

Provider

Invitrogen Antibodies

Product name

VPS11 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: FQQIMHYHM QHEQYRQVIS VCERHGEQDP SLWEQALSYF ARKEEDCKEY VAAVLKHIEN KNLMPPLLVV QTLAHNSTAT LSVIRDYLVQ KLQKQSQQIA QDELRVRRYR EETTRIRQEI QELKASPKIF QKTKCSICNS ALELPSVHFL CGHSFHQHCF ESYSESDADC PTCLPENRKV MDMIRAQEQK RDLHDQFQHQ LRCSNDSFSV IADYFGRGVF NKLTLLTDPP TARLTSSLEA GLQRDLLMHS RRGT (689-941 aa encoded by B C065563)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.17 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human heart tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using VPS11 antibody 19140-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug VPS11 antibody (19140-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-VPS11 (IP:19140-1-AP, 4ug; Detection:19140-1-AP 1:500) with HEK-293 cells lysate 1480ug.