AF3119

antibody from Novus Biologicals

Targeting: GLRX GRX, GRX1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3119

Provider

Novus Biologicals

Product name

Goat Polyclonal Glutaredoxin 1/GLRX1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects endogenous human and mouse Glutaradoxin in Western blots.

Reactivity

Mouse

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Cysteine 111 affects aggregation and cytotoxicity of mutant Cu,Zn-superoxide dismutase associated with familial amyotrophic lateral sclerosis.

Cozzolino M, Amori I, Pesaresi MG, Ferri A, Nencini M, Carrì MT
The Journal of biological chemistry 2008 Jan 11;283(2):866-74

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse Glutaredoxin 1 by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Glutaredoxin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Glutaredoxin 1 at approximately 12 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.