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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- STJA0003841 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-RBFOX3-NeuN antibody [1B7] (STJA0003841)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-FOX3-NeuN is suitable for use in Western Blot and Immunohistochemistry research applications.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C, and avoid repeated freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence of a section of rat brain stem co-labeled with Anti-FOX3 (cat. STJA0003841, green, 1:1000) and Anti-MAP2 (cat. STJA0003676, red, 1:5000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunolabeling of cultured rat neurons showing strong nuclear and distal cytoplasmic labeling with anti-FOX3 (cat. STJA0003841, 1:1000, red). The complete absence of astrocyte staining is shown using anti-GFAP (cat. STJA0003659, 1:1000, green) and nuclear staining was done with DAPI (blue).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
