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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-35954 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR2D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2.
- Concentration
- 1.01 mg/mL
Submitted references Cytoplasmic mRNA decay represses RNA polymerase II transcription during early apoptosis.
Duncan-Lewis C, Hartenian E, King V, Glaunsinger BA
eLife 2021 Jun 4;10
eLife 2021 Jun 4;10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- POLR2D Polyclonal Antibody detects POLR2D protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with POLR2D Polyclonal Antibody (Product # PA5-35954) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- POLR2D Polyclonal Antibody detects POLR2D protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with POLR2D Polyclonal Antibody (Product # PA5-35954) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- POLR2D Polyclonal Antibody detects POLR2D protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: POLR2D protein stained by POLR2D Polyclonal Antibody (Product # PA5-35954) diluted at 1:500. Blue: Hoechst 33342 staining.
