GTX109877

antibody from GeneTex

Targeting: FH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX109877

Provider

GeneTex

Proper citation

GeneTex Cat#GTX109877, RRID:AB_1950283

Product name

Fumarate hydratase antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Fumarase-deficient Uterine Leiomyomas: An Immunohistochemical, Molecular Genetic, and Clinicopathologic Study of 86 Cases.

Miettinen M, Felisiak-Golabek A, Wasag B, Chmara M, Wang Z, Butzow R, Lasota J
The American journal of surgical pathology 2016 Dec;40(12):1661-1669

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Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer.

Lo YW, Lin ST, Chang SJ, Chan CH, Lyu KW, Chang JF, May EW, Lin DY, Chou HC, Chan HL
Journal of cellular and molecular medicine 2015 Apr;19(4):744-59

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Fumarate hydratase antibody detects FH protein by western blot analysis. A. 50 ?g mouse liver lysate/extract 7.5% SDS-PAGE Fumarate hydratase antibody (GTX109877) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Fumarate hydratase antibody detects Fumarate hydratase protein by western blot analysis.A.50 ?g rat liver lysate/extract 10% SDS-PAGEFumarate hydratase antibody (GTX109877) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate)A: HeLaB: HepG2 (GTX27900)10% SDS PAGEGTX109877 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody (GTX109877) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Fumarate hydratase antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: Fumarate hydratase protein stained by Fumarate hydratase antibody (GTX109877) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Fumarate hydratase antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fumarate hydratase protein stained by Fumarate hydratase antibody (GTX109877) diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase (GTX109877) antibody at 1:500 dilution.