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- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- GTX110128 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110128, RRID:AB_1950282
- Product name
- Fumarate hydratase antibody [N2C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Transketolase Regulates the Metabolic Switch to Control Breast Cancer Cell Metastasis via the α-Ketoglutarate Signaling Pathway.
The oncometabolite fumarate promotes pseudohypoxia through noncanonical activation of NF-κB signaling.
Tseng CW, Kuo WH, Chan SH, Chan HL, Chang KJ, Wang LH
Cancer research 2018 Jun 1;78(11):2799-2812
Cancer research 2018 Jun 1;78(11):2799-2812
The oncometabolite fumarate promotes pseudohypoxia through noncanonical activation of NF-κB signaling.
Shanmugasundaram K, Nayak B, Shim EH, Livi CB, Block K, Sudarshan S
The Journal of biological chemistry 2014 Aug 29;289(35):24691-9
The Journal of biological chemistry 2014 Aug 29;289(35):24691-9
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [N2C2], Internal (GTX110128) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fumarate hydratase antibody [N2C2], Internal detects FH protein by western blot analysis. A. 50 ?g mouse liver lysate/extract 10% SDS-PAGE Fumarate hydratase antibody [N2C2], Internal (GTX110128) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: 293T B: A431 (GTX27909) 10% SDS PAGE GTX110128 diluted at 1:5000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fumarate hydratase antibody [N2C2], Internal detects Fumarate hydratase protein by western blot analysis.A.50 ?g rat liver lysate/extract 10% SDS-PAGEFumarate hydratase antibody [N2C2], Internal (GTX110128) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [N2C2], Internal (GTX110128) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using Fumarate hydratase(GTX110128) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fumarate hydratase antibody [N2C2], Internal detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fumarate hydratase protein stained by Fumarate hydratase antibody [N2C2], Internal (GTX110128) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using fumarate hydratase (GTX110128) antibody at 1:500 dilution.
