10966-1-AP

antibody from Invitrogen Antibodies

Targeting: FH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10966-1-AP

Provider

Invitrogen Antibodies

Product name

FH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MYRALRLLA RSRPLVRAPA AALASAPGLG GAAVPSFWPP NAARMASQNS FRIEYDTFGE LKVPNDKYYG AQTVRSTMNF KIGGVTERMP TPVIKAFGIL KRAAAEVNQD YGLDPKIANA IMKAADEVAE GKLNDHFPLV VWQTGSGTQT NMNVNEVISN RAIEMLGGEL GSKIPVHPND HVNKSQSSND TFPTAMHIAA AIEVHEVLLP GLQKLHDALD AKSKEFAQII KIGRTHTQDA VPLTLGQEFS GYVQQVKYAM TRIKAAMPRI YELAAGGTAV GTGLNTRIGF AEKVAAKVAA LTGLPFVTAP NKFEALAAHD ALVELSGAMN TTACSLMKIA NDIRFLGSGP RSGLGELILP ENEPGSSIMP GKVNPTQCEA MTMVAAQVMG NHVAVTVGGS NGHFELNVFK PMMIKNVLHS ARLLGDASVS FTENCVVGIQ ANTERINKLM NESLMLVTAL NPHIGYDKAA KIAKTAHKNG STLKETAIEL GYLTAEQFDE WVKPKDMLGP K (1-510 aa encoded by BC017444)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 10966-1-AP (FH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using FH antibody 10966-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 10966-1-AP (FH Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 10966-1-AP (FH Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer using 10966-1-AP (FH antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HepG2 cells were stained with 0.2ug FH antibody (10966-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-FH (IP:10966-1-AP, 4ug; Detection:10966-1-AP 1:1000) with HepG2 cells lysate 2400ug.