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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP51666PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP51666PU-N, RRID:AB_11149853
- Product name
- anti Fumarase (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the N-terminal region (between 35-63aa) of human Fumarase/FH.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in mouse kidney tissue lysates (35ug/lane) using Fumarase antibody Cat.-No. AP51666PU-N (N-term). This demonstrates the FH antibody detected the FH protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human skin carcinoma (formalin-fixed, paraffin-embedded) using Fumarase/FH antibody Cat.-No. AP51666PU-N, followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FH antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram) using Fumarase/FH antibody Cat.-No. AP51666PU-N. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
