Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 604001 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#604001, RRID:AB_2716194
- Product name
- Purified anti-Dynamin-1
- Antibody type
- Monoclonal
- Reactivity
- Mouse
- Antibody clone number
- 25 µg
- Vial size
- 0.5 mg/ml
- Concentration
- 2°C-8°C
- Storage
- Ambient RT
No comments: Submit comment
Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Total lysates (15 µg protein) from Raji (negative control), A549 and mouse brain were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-Dynamin-1 antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody for anti-Dynamin-1 antibody or HRP Donkey anti-rabbit IgG Antibody for GAPDH antibody. 1:2000 dilution of GAPDH (poly6314) antibody was used as a loading control (lower). Lane M: MW ladder.
- Conjugate
- Unconjugated