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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- TA347137 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Hira Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Hira Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- HIRA
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- not determined
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) with the antibody against mouse Hira, diluted 1:1,000 in BSA/PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (112 kDa) is indicated on the right.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against mouse Hira. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:6,300.
- Validation comment
- ELISA
