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LearnNB100-1452
antibody from Novus Biologicals
Targeting: TXNDC5
EndoPDI, ERp46, FLJ21353, FLJ90810, Hcc-2, MGC3178, PDIA15
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB100-1452 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1452, RRID:AB_2212317
- Product name
- Goat Polyclonal TXNDC5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to be able to recognize both reported human isoforms, as represented by NP_110437.2
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references EndoPDI, a novel protein-disulfide isomerase-like protein that is preferentially expressed in endothelial cells acts as a stress survival factor.
Sullivan DC, Huminiecki L, Moore JW, Boyle JJ, Poulsom R, Creamer D, Barker J, Bicknell R
The Journal of biological chemistry 2003 Nov 21;278(47):47079-88
The Journal of biological chemistry 2003 Nov 21;278(47):47079-88
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TXNDC5 Antibody [NB100-1452] - (0.1ug/ml) of human placenta lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TXNDC5 Antibody [NB100-1452] - Staining of HEK293 (A), A549 (B), HeLa (C) with antibody at 0.1 ug/mL and of HepG2 (D) cell lysate with antibody at 0.01 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TXNDC5 Antibody [NB100-1452] - Staining of paraffin embedded Human Small Intestine. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TXNDC5 Antibody [NB100-1452] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
