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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB0256 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0256, RRID:AB_1019031
- Product name
- PTPN13 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant PTPN13.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Expression and potential role of Fas-associated phosphatase-1 in ovarian cancer.
Meinhold-Heerlein I, Stenner-Liewen F, Liewen H, Kitada S, Krajewska M, Krajewski S, Zapata JM, Monks A, Scudiero DA, Bauknecht T, Reed JC
The American journal of pathology 2001 Apr;158(4):1335-44
The American journal of pathology 2001 Apr;158(4):1335-44
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PTPN13. Using PTPN13 polyclonal antibody (Cat # PAB0256) at 1 : 2000. In ovarian carcinoma cell lines, PTPN13 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human Jurkat T and 293 kidney cell lines were used as negative and positive controls, respectively.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of PTPN13 in cell lines. Using PTPN13 polyclonal antibody (Cat # PAB0256) at 1 : 2000. In ovarian carcinoma cell lines PTPN13 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human 293 kidney and Jurkat T cell lines were used as positive and negative controls, respectively. The staining data correlates with the western blot data (figure to the left) .
- Validation comment
- Immunocytochemistry
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemisty of PTPN13 in formalin-fixed, paraffin embedded ovarian carcinoma cores from a tissue microarray. Using PTPN13 polyclonal antibody (Cat # PAB0256) at 1 : 2000. A-D, samples are from four different patients. A1-D1 are high magnification images from A-D, respectively. Hematoxylin-eosin counterstain.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
