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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-72906 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTPN13 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Calpain-1 and Calpain-2 in the Brain: New Evidence for a Critical Role of Calpain-2 in Neuronal Death.
Wang Y, Liu Y, Bi X, Baudry M
Cells 2020 Dec 16;9(12)
Cells 2020 Dec 16;9(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTPN13 in ovarian carcinoma cell lines PTPN13/PTPL1 . Samples were incubated in PTPN13 polyclonal antibody (Product # PA5-72906). Expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human Jurkat T and 293 kidney cell lines were used as negative and positive controls, respectively.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PTPN13 in Ovarian carcinoma cores from a tissue microarray. Samples were incubated in PTPN13 polyclonal antibody (Product # PA5-72906) using a dilution of 1:2000. A-D, samples are from four different patients. A1-D1 are high magnification images from A-D, respectively. Hematoxylin-eosin counterstain.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PTPN13 in ovarian carcinoma cell lines. Samples were incubated in PTPN13 polyclonal antibody (Product # PA5-72906) using a dilution of 1:2000. Expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human 293 kidney and Jurkat T cell lines were used as positive and negative controls, respectively.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PTPN13 in Ovarian carcinoma cores from a tissue microarray. Samples were incubated in PTPN13 polyclonal antibody (Product # PA5-72906) using a dilution of 1:2000. A-D, samples are from four different patients. A1-D1 are high magnification images from A-D, respectively. Hematoxylin-eosin counterstain.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Changes in spectrin, striatal-enriched tyrosine phosphatase (STEP), PTPN13 breakdown product (P13BP), and TAR DNA-binding protein of about 43 kDa (TDP-43) in brain of WT, calpain-1 knock out (C1KO), and C2CKO mice 24 h after traumatic brain injury (TBI). ( A , B ) Representative western blots (WBs) of ipsilateral cortical tissue (P2 fraction) from WT, C1KO, and C2CKO mice collected 24 h after TBI or sham surgery. Note that calpain-1 is absent in C1KO mice and calpain-2 levels were reduced in C2CKO mice. Several calpain substrates including spectrin (1:500; MAB1622, EMD Millipore, Burlington, MA, USA), STEP (1:1000, NB300-202, Novus Biologicals, Colorado, CO, USA), PTPN13 (1:1000, PA5-72906, Thermo Fisher Scientific, Waltham, MA, USA), and TDP-43 (1:1000, 10782-2-AP, Proteintech, Chicago, IL, USA) were probed, and their fragments generated by calpain cleavage including SBDP, STEP33, and P13BPs were detected after TBI. ( C ) Quantification of WB. Ratios of SBDPs to spectrin, STEP33 to STEP61, P13BPs to PTPN13 were significantly increased in WT and C1KO but not in C2CKO mice after TBI. Levels of TDP-43 were not changed after TBI in all genotypes. * p < 0.05, ** p < 0.01. ns, no significant difference. N = 4. One way ANOVA followed by Bonferroni's test. Full immunoblots for the blots shown here are presented in the Supplementary Materials .
