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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP21792SU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21792SU-S, RRID:AB_10757410
- Product name
- anti PTPN13
- Antibody type
- Polyclonal
- Antigen
- A recombinant protein corresponding to amino acids 1279 to 1883 of human FAP-1
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 50 µl
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of FAP-1 using AP21792SU-S at 1/2000. In ovarian carcinoma cell lines FAP-1 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human Jurkat T and 293 kidney cell lines were used as negative and positive controls, respectively.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of FAP-1 in cell lines using AP21792SU-S at 1/2000. In ovarian carcinoma cell lines FAP-1 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human 293 kidney and Jurkat T cell lines were used as positive and negative controls, respectively. The staining data correlates with the western blot data (figure to the left).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemisty of FAP-1 in formalin-fixed, paraffin embedded ovarian carcinoma cores from a tissue microarray using AP21792SU-S at 1/2000. A-D, samples are from four different patients. A1-D1 are high magnification images from A-D, respectively. Hematoxylin-eosin counterstain.
